The centre's activities are divided into four main parts: theatre, music, musical theatre and a programme for children. All are available on a subscription basis. At the centre, one can see theatre performances by local groups as well as touring performances by Slovene theatre houses such as SNG Drama Ljubljana, Ljubljana City Theatre (MGL) or SNG Nova Gorica as well as performances from domestic and international musicians with Slovene Philharmonic Orchestra, Bill Evans and Mitteleuropa Orchestra amongst others. The centre also serves as a venue for jazz concerts which are part of Jazzinty international summer jazz festival and musical workshop that has been taking place annually in the historic city centre of Novo mesto for twenty years.
The main hall has a capacity of 366 seats and is suitable for concert and dance performances, technically less demanding theatre plays, film screenings, conferences and meetings. The Janez Trdina Cultural Centre's hall can also be rented.
Within the venue, there is also an exhibition space with 80 seats available. It is suitable for smaller-scale performances and exhibitions as well as banquets and receptions. In 2019, it hosted an ongoing concert series called Kocka Out of the Box Sessions which presented a diverse selection of music performed in an intimate setting.